Continuous-flow electrophoresis for concentration of protein.
نویسندگان
چکیده
منابع مشابه
Simple method for concentration of cerebrospinal fluid for protein electrophoresis.
MOST CLINICAL LABORATORIES use paper electrophoresis for the separahon and quantitation of serum proteins. There has been increasing iiiterest in the fractionation of proteins Ill other body fluids, particularly cerebrospinal fluid. Successful paper electrophoresis requires a high protein concentration in the fluid applied to the l)POF strips, If tile total protein content of spinal fluid is no...
متن کاملPreparation of native tobacco mosaic virus protein by continuous free-flow electrophoresis.
diesem Grunde ist es auch verfrüht, bei der Hem mung des Citronensäurezyklus in den S-Mangelblättern direkt eine Hemmung etwa der Bildung von Coenzym A anzunehmen. Doch deuten die Beob achtungen daraufhin, daß primär nicht die Photo synthese durch den S-Mangel getroffen ist, sondern die Atmung. Da unsere Untersuchungen zum Proteinumsatz unter S-Mangel ergaben, daß der S-Gehalt des Cyto plas...
متن کاملEnzyme fractionation by means of large-scale continuous-flow electrophoresis.
Large-scale, microglass bead, continuous-flow electrophoresis was used to separate and collect protein fractions from a single serum sample. These fractions were assayed for a multiplicity of enzyme activities. Relatively good recovery was obtained with lactic acid dehydrogenase, leucine aminopeptidase, and phosphohexoseisomerase. Artifactually high recoveries were observed with aldotase and is...
متن کاملKinematic Stabilization of Continuous-flow Electrophoresis against Thermal Convection.
The Problem of Thermal Convection.-Electrophoresis presupposes the m ain tenance of an electric field in a conductive fluid (usually a buffer solution). The electric field dV/dx cannot be maintained without a concomitant current density J2 = u(dV/dx). In an electrophoretic column of uniform cross section, the heat generated in a unit volume per unit time will thus be: dQ/dt = Jxlla = (dV/dx)2a....
متن کاملFrontal analysis continuous capillary electrophoresis for protein-polyelectrolyte binding studies.
A novel technique, frontal analysis continuous capillary electrophoresis (FACCE), has been described as an effective way to study protein-polyelectrolyte binding. FACCE involves continuous sampling, integrating sample injection and separation into one process that provides advantages over conventional frontal chromatography. The method provides rapid and precise determination of binding isother...
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ژورنال
عنوان ژورنال: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
سال: 1991
ISSN: 0021-9592,1881-1299
DOI: 10.1252/jcej.24.62